Steatohepatitis Impairs T-cell-Directed Immunotherapies Against Liver Tumors in Mice.

ABSTRACT:

Background & aims

Nonalcoholic steatohepatitis causes loss of hepatic CD4⁺ T cells and promotes tumor growth. The liver is the most common site of distant metastases from a variety of malignancies, many of which respond to immunotherapy. We investigated the effects of steatohepatitis on the efficacy of immunotherapeutic agents against liver tumors in mice.

Methods

Steatohepatitis was induced by feeding C57BL/6NCrl or BALB/c AnNCr mice a methionine and choline-deficient diet or a choline-deficient l-amino acid-defined diet. Mice were given intrahepatic or subcutaneous injections of B16 melanoma and CT26 colon cancer cells, followed by intravenous injections of M30-RNA vaccine (M30) or intraperitoneal injections of an antibody against OX40 (aOX40) on days 3, 7, and

SUBMITTER: Heinrich B 

PROVIDER: S-EPMC7755834 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature