Interferon Regulatory Factor 5 Mediates Lipopolysaccharide-Induced Neuroinflammation.

ABSTRACT:

Background

Activated microglia play a vital role in neuroinflammation in the central nervous system (CNS), which is associated with the pathogenesis and the progression of neurological diseases. Interferon regulatory factor 5 (IRF5) has been well established participating in inflammatory responses and is highly expressed in M1 macrophage in the periphery, the role of which in the CNS remains elusive.

Methods

Lipopolysaccharide (LPS) was employed to induce neuroinflammation. Down-regulation of IRF5 in C57/BL6 mice and BV2 microglial cells were achieved by IRF5 siRNA transfection. The levels of pro-inflammatory cytokines were evaluated by ELISA and quantitative real-time PCR. The expression levels of IRF5 were examined by immunofluorescence and Western blot.

Results

LPS

SUBMITTER: Fan Z 

PROVIDER: S-EPMC7755991 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature