Non-full-length Water-Soluble CXCR4^QTY and CCR5^QTY Chemokine Receptors: Implication for Overlooked Truncated but Functional Membrane Receptors.

ABSTRACT: It was posited that functionalities of GPCRs require full-length sequences that are negated by residue deletions. Here we report that significantly truncated nfCCR5^QTY and nfCXCR4^QTY still bind native ligands. Receptor-ligand interactions were discovered from yeast 2-hybrid screening and confirmed by mating selection. Two nfCCR5^QTY (SZ218a, SZ190b) and two nfCXCR4^QTY (SZ158a, SZ146a) were expressed in E. coli. Synthesized receptors exhibited α-helical structures and bound respective ligands with reduced affinities. SZ190b and SZ158a were reconverted into non-QTY forms and expressed in HEK293T cells. Reconverted receptors localized on cell membranes and functioned as negative regulators for ligand-induced signaling when co-expressed with fu

SUBMITTER: Qing R 

PROVIDER: S-EPMC7756140 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature