The Impact of Halogenated Phenylalanine Derivatives on NFGAIL Amyloid Formation.

ABSTRACT: The hexapeptide hIAPP_22-27 (NFGAIL) is known as a crucial amyloid core sequence of the human islet amyloid polypeptide (hIAPP) whose aggregates can be used to better understand the wild-type hIAPP's toxicity to β-cell death. In amyloid research, the role of hydrophobic and aromatic-aromatic interactions as potential driving forces during the aggregation process is controversially discussed not only in case of NFGAIL, but also for amyloidogenic peptides in general. We have used halogenation of the aromatic residue as a strategy to modulate hydrophobic and aromatic-aromatic interactions and prepared a library of NFGAIL variants containing fluorinated and iodinated phenylalanine analogues. We used thioflavin T staining, transmission electron microscopy (TEM) and small-angle X-ray s

SUBMITTER: Chowdhary S 

PROVIDER: S-EPMC7756607 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature