Genome-wide functional analysis of hot pepper immune receptors reveals an autonomous NLR clade in seed plants.

ABSTRACT: Plants possess hundreds of intracellular immune receptors encoding nucleotide-binding domain leucine-rich repeat (NLR) proteins. Full-length NLRs or a specific domain of NLRs often induce plant cell death in the absence of pathogen infection. In this study we used genome-wide transient expression analysis to identify a group of NLRs (ANLs; ancient and autonomous NLRs) carrying autoactive coiled-coil (CC^A ) domains in pepper (Capsicum annuum). CC^A -mediated cell death mimics hypersensitive cell death triggered by the interaction between NLRs and pathogen effectors. Sequence alignment and mutagenesis analyses revealed that the intact α1 helix of CC^A s is critical for both CC^A - and ANL-mediated cell death. Cell death induced by CC^A s doe

SUBMITTER: Lee HY 

PROVIDER: S-EPMC7756659 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature