Co-staining of K_Ca 3.1 Channels in NSCLC Cells with a Small-Molecule Fluorescent Probe and Antibody-Based Indirect Immunofluorescence.

ABSTRACT: The Ca²⁺ activated potassium channel 3.1 (K_Ca 3.1) is involved in critical steps of the metastatic cascade, such as proliferation, migration, invasion and extravasation. Therefore, a fast and efficient protocol for imaging of K_Ca 3.1 channels was envisaged. The novel fluorescently labeled small molecule imaging probes 1 and 2 were synthesized by connecting a dimethylpyrrole-based BODIPY dye with a derivative of the K_Ca 3.1 channel inhibitor senicapoc via linkers of different length. Patch-clamp experiments revealed the inhibition of K_Ca 3.1 channels by the probes confirming interaction with the channel. Both probes 1 and 2 were able to stain K_Ca 3.1 channels in non-small-cell lung cancer (NSCLC) cells following a simple, fas

SUBMITTER: Brommel K 

PROVIDER: S-EPMC7756743 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature