CAFs affect the proliferation and treatment response of head and neck cancer spheroids during co-culturing in a unique in vitro model.

ABSTRACT:

Background

Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous group of tumors for which the overall survival rate worldwide is around 60%. The tumor microenvironment, including cancer-associated fibroblasts (CAFs), is believed to affect the treatment response and migration of HNSCC. The aim of this study was to create a biologically relevant HNSCC in vitro model consisting of both tumor cells and CAFs cultured in 3D to establish predictive biomarkers for treatment response, as well as to investigate the impact of CAFs on phenotype, proliferation and treatment response in HNSCC cells.

Methods

Three different HNSCC patient-derived tumor cell lines were cultured with and without CAFs in a 3D model. Immunohistochemistry of the proliferation marker Ki67, epidermal g

SUBMITTER: Magan M 

PROVIDER: S-EPMC7756959 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature