Effect of claudin 1 on cell proliferation, migration and apoptosis in human cervical squamous cell carcinoma.

ABSTRACT: Claudin 1 is a member of the claudin protein family that serves an important role in tight junctions. Increased or decreased expression levels of claudin 1 are found in several diseases, including breast cancer and viral infections. However, the function of claudin 1 in cervical cancer remains controversial. The aim of the present study was to investigate the biological functions of claudin 1 in different human cervical cancer cell lines. First, SiHa and ME‑180 cells with stable claudin 1 overexpression or knockdown were established using lentiviral transduction, and the mRNA and protein levels were measured via reverse transcription‑quantitative PCR and western blot analysis. Subsequently, cell proliferation, colony formation and migration experiments were performed in vitro using standar

SUBMITTER: Shen Z 

PROVIDER: S-EPMC7757103 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature