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Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells.

ABSTRACT: This protocol offers a detailed procedure for the in vitro differentiation of human pluripotent stem cells (hPSCs) to multipotent hematopoietic progenitors that arise from SOX17⁺ hemogenic endothelium, mimicking intra-embryonic, HOXA-positive, aorta-gonad mesonephros (AGM) hematopoiesis. The generated endothelium displays transcriptional similarities to cells sorted from human 5-week AGM, and CD45⁺CD34⁺RUNX1C⁺ progenitors share an accessible chromatin profile with adult hematopoietic stem cells and multipotent progenitors. Therefore, this protocol is suitable for the mechanistic study of human multipotent progenitor development and for modeling childhood leukemias. For complete details on the use and execution of this protocol, please refer to Nafria et al. (2020).

SUBMITTER: Nafria M

PROVIDER: S-EPMC7757115 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells.

Nafria Monica M Bonifer Constanze C Stanley Edouard Guy EG Ng Elizabeth Siewsun ES Elefanty Andrew George AG

STAR protocols 20201016 3

This protocol offers a detailed procedure for the in vitro differentiation of human pluripotent stem cells (hPSCs) to multipotent hematopoietic progenitors that arise from SOX17+ hemogenic endothelium, mimicking intra-embryonic, HOXA-positive, aorta-gonad mesonephros (AGM) hematopoiesis. The generated endothelium displays transcriptional similarities to cells sorted from human 5-week AGM, and CD45+CD34+RUNX1C+ progenitors share an accessible chr ...[more]

PMID: 33377024

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