New Insights into the Phage Genetic Switch: Effects of Bacteriophage Lambda Operator Mutations on DNA Looping and Regulation of P_R, P_L, and P_RM.

ABSTRACT: One of the best understood systems in genetic regulatory biology is the so-called "genetic switch" that determines the choice the phage-encoded CI repressor binds cooperatively to tripartite operators, O_L and O_R, in a defined pattern, thus blocking the transcription at two lytic promoters, P_L and P_R, and auto-regulating the promoter, P_RM, which directs CI synthesis by the prophage. Fine-tuning of the maintenance of lysogeny is facilitated by interactions between CI dimers bound to O_R and O_L through the formation of a loop by the intervening DNA segment. By using a purified in vitro transcription system, we have genetically dissected the roles of individual operator sites in the formation of the DNA loop and thus h

SUBMITTER: Lewis DEA 

PROVIDER: S-EPMC7757127 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature