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In Vitro Derivation of Quiescent Mouse Embryonic Stem Cells Based on Distinct Mitochondrial Activity.

ABSTRACT: Embryonic diapause is a naturally occurring strategy in mammals that determines successful rates of gestation under unfavorable conditions. This dormant state can be captured in the form of quiescent mouse embryonic stem cells (ESCs). Here, we present a step-by-step protocol to derive quiescent ESCs that naturally exist in culture by harnessing the heterogeneity of mitochondrial activity. The derived quiescent ESCs with low mitochondrial activity can be utilized as a surrogate to study stages of early embryonic development. For complete details on the use and execution of this protocol, please refer to Khoa et al. (2020).

SUBMITTER: Khoa LTP

PROVIDER: S-EPMC7757300 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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<i>In Vitro</i> Derivation of Quiescent Mouse Embryonic Stem Cells Based on Distinct Mitochondrial Activity.

Khoa Le Tran Phuc LTP Dou Yali Y

STAR protocols 20201020 3

Embryonic diapause is a naturally occurring strategy in mammals that determines successful rates of gestation under unfavorable conditions. This dormant state can be captured in the form of quiescent mouse embryonic stem cells (ESCs). Here, we present a step-by-step protocol to derive quiescent ESCs that naturally exist in culture by harnessing the heterogeneity of mitochondrial activity. The derived quiescent ESCs with low mitochondrial activity can be utilized as a surrogate to study stages of ...[more]

PMID: 33377030

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