Determining Alternative Protein Isoform Expression Using RNA Sequencing and Mass Spectrometry.
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ABSTRACT:
SUBMITTER: Han Y
PROVIDER: S-EPMC7757315 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Publications
Determining Alternative Protein Isoform Expression Using RNA Sequencing and Mass Spectrometry.
Han Yu Y Wright Julianna M JM Lau Edward E Lam Maggie Pui Yu MPY
STAR protocols 20201021 3
Alternative splicing greatly expands the coding capacity of the human genome, but how many alternative transcripts are translated as proteins or carry functional importance remains unknown and awaits experimental verification. Here, we describe a protocol that combines transcriptomics (RNA-seq) and proteomics (mass spectrometry [MS]) analyses to identify alternative isoforms in proteomes. This workflow is applicable to custom-generated RNA-seq and MS data from matching samples, as well as the re ...[more]
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