Protocol for Monitoring DNA-Triggered cGAS/STING Signaling in Mammalian Cells and Mice.

ABSTRACT: Innate immunity is the first layer of defense against infection in mammals and is tightly regulated. We monitored cGAS/STING signaling upon ISD90 or 2',3'-cGAMP stimulation in EA.hy926 cells by western blotting, RT-PCR, and ELISA analyses to reveal signaling activation and IFNβ production. In addition, we also include an HSV-1 infected mouse model to further reveal procedures in analyzing cGAS/STING signaling in mice. This protocol could be applied to studies focusing on cell culture or mouse models to investigate cGAS/STING signaling. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2020).

SUBMITTER: Wang Y 

PROVIDER: S-EPMC7757363 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature