An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis.
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ABSTRACT:
SUBMITTER: Kasashima H
PROVIDER: S-EPMC7757567 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
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An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis.
Kasashima Hiroaki H Duran Angeles A Cid-Diaz Tania T Kudo Yotaro Y Diaz-Meco Maria T MT Moscat Jorge J
STAR protocols 20201120 3
This protocol provides the steps required for a mouse liver orthotopic implantation model. The reliable pre-clinical animal models that have similar characteristics to hepatocellular carcinoma (HCC) are a powerful tool to unveil the mechanisms controlling tumor initiation and progression. Here, we describe a syngeneic orthotopic HCC model that recapitulates the role of a host pro-tumorigenic microenvironment by pre-conditioning mouse livers with a high-fat diet (HFD). For complete details on the ...[more]
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