Highly Multiplex Targeted Proteomics Enabled by Real-Time Chromatographic Alignment.

ABSTRACT: Targeted mass spectrometry methods produce high-quality quantitative data in terms of limits of detection and dynamic range, at the cost of a substantial compromise in throughput compared to methods such as data independent and data dependent acquisition. The logistical and experimental issues inherent to maintaining assays of even several hundred targets are significant. Prominent among these issues is the drift in analyte retention time as liquid chromatography (LC) columns wear, forcing targeted scheduling windows to be much larger than LC peak widths. If these problems could be solved, proteomics assays would be capable of targeting thousands of peptides in an hour-long experiment, enabling large cohort studies to be performed without sacrificing sensitivity and specificity. We describ

SUBMITTER: Remes PM 

PROVIDER: S-EPMC7757911 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature