Bud23 promotes the final disassembly of the small subunit Processome in Saccharomyces cerevisiae.

ABSTRACT: The first metastable assembly intermediate of the eukaryotic ribosomal small subunit (SSU) is the SSU Processome, a large complex of RNA and protein factors that is thought to represent an early checkpoint in the assembly pathway. Transition of the SSU Processome towards continued maturation requires the removal of the U3 snoRNA and biogenesis factors as well as ribosomal RNA processing. While the factors that drive these events are largely known, how they do so is not. The methyltransferase Bud23 has a role during this transition, but its function, beyond the nonessential methylation of ribosomal RNA, is not characterized. Here, we have carried out a comprehensive genetic screen to understand Bud23 function. We identified 67 unique extragenic bud23Δ-suppressing mutations that mapped to ge

SUBMITTER: Black JJ 

PROVIDER: S-EPMC7758049 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature