PET Imaging of Neutrophils Infiltration in Alzheimer's Disease Transgenic Mice.

ABSTRACT: Neutrophils are important components in the innate immune system. Neutrophil hyperactivation is regarded as a characteristic of Alzheimer's disease (AD). But in vivo imaging tools observing neutrophil activity in AD dynamically is lacking. This study aimed to identify neutrophil infiltration in AD transgenic mice. We used the AD triple-mutant transgenic mouse model and identified the genotype with RT-PCR. Behavioral experiments including an open-field test, a Morris water maze, and a Y-maze test were performed to evaluate the status of this AD model. ¹⁸F-AV45, ¹⁸F-PM-PBB3, ⁶⁸Ga-PEG-cFLFLFK, and ¹⁸F-DPA714 were synthesized according to previous reports. We employed microPET to detect tracer uptake in the AD model and the control mice at dif

SUBMITTER: Kong Y 

PROVIDER: S-EPMC7758535 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature