Caffeine and Theophylline Inhibit β-Galactosidase Activity and Reduce Expression in Escherichia coli.

ABSTRACT: The β-galactosidase enzyme is a common reporter enzyme that has been used extensively in microbiological and synthetic biology research. Here, we demonstrate that caffeine and theophylline, common natural methylxanthine products found in many foods and pharmaceuticals, negatively impact both the expression and activity of β-galactosidase in Escherichia coli. The β-galactosidase activity in E. coli grown with increasing concentrations of caffeine and theophylline was reduced over sixfold in a dose-dependent manner. We also observed decreasing lacZ mRNA transcript levels with increasing methylxanthine concentrations in the growth media. Similarly, caffeine and theophylline inhibit the activity of the purified β-galactosidase enzyme, with an approximately 1.7-fold increas

SUBMITTER: Horne J 

PROVIDER: S-EPMC7758883 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature