Indirect cholinergic activation slows down pancreatic cancer growth and tumor-associated inflammation.

ABSTRACT:

Background

Nerve-cancer interactions are increasingly recognized to be of paramount importance for the emergence and progression of pancreatic cancer (PCa). Here, we investigated the role of indirect cholinergic activation on PCa progression through inhibition of acetylcholinesterase (AChE) via clinically available AChE-inhibitors, i.e. physostigmine and pyridostigmine.

Methods

We applied immunohistochemistry, immunoblotting, MTT-viability, invasion, flow-cytometric-cell-cycle-assays, phospho-kinase arrays, multiplex ELISA and xenografted mice to assess the impact of AChE inhibition on PCa cell growth and invasiveness, and tumor-associated inflammation. Survival analyses were performed in a novel genetically-induced, surgically-resectable mouse model of PCa under adjuvant tr

SUBMITTER: Pfitzinger PL 

PROVIDER: S-EPMC7758936 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature