Constructive Optimization of a Multienzymatic Film Based on a Cascade Reaction for Electrochemical Biosensors.

ABSTRACT: The application of a multienzyme cascade reaction in electrochemical biosensors has the advantage of expanding the target substrates in addition to selectivity combining multiple enzymes on an electrode. However, the multienzyme system has the drawback of inefficient substance conversion because of the time-consuming passing of intermediates between the enzymes and/or diffusional loss of the intermediates. In this study, the optimal construction of a multienzymatic film in an ammonia detection sensor was investigated using a cascade reaction of l-glutamate oxidase and l-glutamate dehydrogenase as a model sensor. Three enzymatic films were prepared: (1) a mixed film designed to have a short diffusional distance between closely located enzymes, (2) a normal-sequential layered film arranged f

SUBMITTER: Sasaki K 

PROVIDER: S-EPMC7758940 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature