Characterization of Human Salivary Extracellular RNA by Next-generation Sequencing.

ABSTRACT:

Background

It was recently discovered that abundant and stable extracellular RNA (exRNA) species exist in bodily fluids. Saliva is an emerging biofluid for biomarker development for noninvasive detection and screening of local and systemic diseases. Use of RNA-Sequencing (RNA-Seq) to profile exRNA is rapidly growing; however, no single preparation and analysis protocol can be used for all biofluids. Specifically, RNA-Seq of saliva is particularly challenging owing to high abundance of bacterial contents and low abundance of salivary exRNA. Given the laborious procedures needed for RNA-Seq library construction, sequencing, data storage, and data analysis, saliva-specific and optimized protocols are essential.

Methods

We compared different RNA isolation methods and library con

SUBMITTER: Li F 

PROVIDER: S-EPMC7759158 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature