Altered N-glycan composition impacts flagella-mediated adhesion in Chlamydomonas reinhardtii.

ABSTRACT: For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion. However, it is not known whether only the presence or also the composition of N-glycans attached to respective proteins is important for these processes. To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions. Taking advantage of atomic force microscopy and micropipette force measurements, our data revealed that reduction in N-glycan complexity impedes the adhesion force required for binding the flagella to surfaces. This results in i

SUBMITTER: Xu N 

PROVIDER: S-EPMC7759384 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature