Targeted Transgene Expression in Rice Using a Callus Strong Promoter for Selectable Marker Gene Control.

ABSTRACT: Precise expression of a transgene in the desired manner is important for plant genetic engineering and gene function deciphering, but it is a challenge to obtain specific transgene expression free from the interference of the constitutive promoters used to express the selectable marker gene, such as the Cauliflower mosaic virus (CaMV) 35S promoter. So, the solutions to avoid these inappropriate regulations are largely demanded. In this study, we report the characterization of a callus strong promoter (CSP1) in rice and its application for accurate transgene expression. Our results indicate that the high expression of the CSP1 promoter in the callus enables efficient selection of hygromycin equivalent to that provided by the CaMV 35S promoter, whereas its expression in other t

SUBMITTER: Zhou J 

PROVIDER: S-EPMC7759479 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature