Evaluating ELISA, Immunofluorescence, and Lateral Flow Assay for SARS-CoV-2 Serologic Assays.

ABSTRACT:

Background

The SARS-CoV-2 outbreak has emerged at the end of 2019. Aside from the detection of viral genome with specific RT-PCR, there is a growing need for reliable determination of the serological status. We aimed at evaluating five SARS-CoV-2 serology assays.

Methods

An in-house immunofluorescence assay (IFA), two ELISA kits (EUROIMMUN^® ELISA SARS-CoV-2 IgG and NovaLisa^® SARS-CoV-2 IgG and IgM) and two lateral flow assays (T-Tek^® SARS-CoV-2 IgG/IgM Antibody Test Kit and Sure Bio-tech^® SARS-CoV-2 IgM/IgG Antibody Rapid Test) were compared on 40 serums from RT-PCR-confirmed SARS-CoV-2 infected patients and 10 SARS-CoV-2 RT-PCR negative subjects as controls.

Results

Control subjects tested negative for SARS-CoV-2 antibodies

SUBMITTER: Michel M 

PROVIDER: S-EPMC7759487 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature