Thioamide quenching of intrinsic protein fluorescence.

ABSTRACT: Thioamides quench tryptophan and tyrosine fluorescence in a distance-dependent manner and thus can be used to monitor the binding of thioamide-containing peptides to proteins. Since thioamide analogs of the natural amino acids can be synthetically incorporated into peptides, they can function as minimally-perturbing probes of protein/peptide interactions.

SUBMITTER: Goldberg JM 

PROVIDER: S-EPMC7759719 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature