LAMP-LFD Based on Isothermal Amplification of Multicopy Gene <i>ORF160b</i>: Applicability for Highly Sensitive Low-Tech Screening of Allergenic Soybean (<i>Glycine max</i>) in Food.

ABSTRACT: Soybean (Glycine max) allergy can be life threatening. A lack of causative immunotherapy of soybean allergy makes soybean avoidance indispensable. Detection methods are essential to verify allergen labeling and unintentional allergen cross contact during food manufacture. Here, we aimed at evaluating our previously described primers for loop-mediated isothermal amplification (LAMP) of multicopy gene ORF160b, combined with a lateral flow dipstick (LFD)-like detection, for their performance of soybean detection in complex food matrices. The results were compared with those obtained using quantitative real-time Polymerase Chain Reaction (qPCR) as the current standard of DNA-based allergen detection, and antibody-based commercial lateral flow device (LFD) as the current reference

SUBMITTER: Allgower SM 

PROVIDER: S-EPMC7760099 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature