Quantification of Drugs in Distinctly Separated Ocular Substructures of Albino and Pigmented Rats.

ABSTRACT: The rat is a commonly used species in ocular drug research. Detailed methods of separating rat ocular tissues have not been described in literature. To understand the intraocular drug distribution, we developed a robust method for the separation of individual anterior and posterior substructures of pigmented Brown Norway (BN) and albino Wistar Han (WH) rat eyes, followed by quantification of drug concentration in these substructures. A short formalin incubation, which did not interfere with drug quantification, enabled the preservation of individual tissue sections while minimizing cross-tissue contamination, as demonstrated by histological analysis. Following oral administration, we applied the tissue separation method, in order to determine the ocular concentrations of dexamethasone and

SUBMITTER: Rimpela AK 

PROVIDER: S-EPMC7760391 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature