Peptide Linker Affecting the Activity Retention Rate of VHH in Immunosorbents.

ABSTRACT: VHH-based immunosorbents are an emerging and promising tool for the removal of toxic substances from plasma. However, the small size of VHHs is a double-edged sword, bringing both benefits and drawbacks to the immunosorbent. The small size of the VHH allows a higher coupling density, while the closer distance to the resin might create steric hindrance for paratope access. The latter could be avoided by inserting a linker between the VHH and the gel attachment site. Here, we report an approach to improve the activity retention of the immobilized VHH by selecting suitable linkers between the VHH and the site-specific immobilization site on the resin. Seven peptide linkers differing in length and flexibility were fused to the VHH and contained the formylglycine generating enzyme (FGE) recogni

SUBMITTER: Li D 

PROVIDER: S-EPMC7760621 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature