Construction of a Nanosensor for Non-Invasive Imaging of Hydrogen Peroxide Levels in Living Cells.

ABSTRACT: Hydrogen peroxide (H₂O₂) serves fundamental regulatory functions in metabolism beyond the role as damage signal. During stress conditions, the level of H₂O₂ increases in the cells and causes oxidative stress, which interferes with normal cell growth in plants and animals. The H₂O₂ also acts as a central signaling molecule and regulates numerous pathways in living cells. To better understand the generation of H₂O₂ in environmental responses and its role in cellular signaling, there is a need to study the flux of H₂O₂ at high spatio-temporal resolution in a real-time fashion. Herein, we developed a genetically encoded Fluorescence Resonance Energy Transfer (FRET)-based nanosensor (FL

SUBMITTER: Amreen 

PROVIDER: S-EPMC7760702 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature