Innovative Animal Model of DSS-Induced Ulcerative Colitis in Pseudo Germ-Free Mice.

ABSTRACT: The aim of this study was to investigate the use of a standardized animal model subjected to antibiotic treatment, and the effects of this treatment on the course of dextran sodium sulphate (DSS)-induced colitis in mice. By decontamination with selective antibiotics and observation of pathogenesis of ulcerative colitis (UC) induced chemically by exposure of mice to various concentrations of DSS, we obtained an optimum animal PGF model of acute UC manifested by mucin depletion, epithelial degeneration and necrosis, leading to the disappearance of epithelial cells, infiltration of lamina propria and submucosa with neutrophils, cryptitis, and accompanied by decreased viability of intestinal microbiota, loss of body weight, dehydration, moderate rectal bleeding, and a decrease in the selected

SUBMITTER: Gancarcikova S 

PROVIDER: S-EPMC7761014 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature