The Genetic Evidence of Burn-Induced Cardiac Mitochondrial Metabolism Dysfunction.

ABSTRACT: Burn-induced cardiac dysfunction is thought to involve mitochondrial dysfunction, although the mechanisms responsible are unclear. In this study, we used our established model of in vivo burn injury to understand the genetic evidence of burn-induced mitochondrial confusion dysfunction by describing cardiac mitochondrial metabolism-related gene expression after burn. Cardiac tissue was collected at 24 hours after burn injury. An O2K respirometer system was utilized to measure the cardiac mitochondrial function. Oxidative phosphorylation complex activities were determined using enzyme activity assays. RT Profiler PCR array was used to identify the differential regulation of genes involved in mitochondrial biogenesis and metabolism. The quantitative qPCR and Western blotting were applied to v

SUBMITTER: Wen JJ 

PROVIDER: S-EPMC7761708 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature