Optimization of Polyhydroxybutyrate Production by Amazonian Microalga Stigeoclonium sp. B23.

ABSTRACT: The present work established the optimization and production of biodegradable thermoplastic polyhydroxybutyrate (PHB) from Amazonian microalga Stigeoclonium sp. B23. The optimization was performed in eight different growth media conditions of Stigeoclonium sp. B23, supplemented with sodium acetate and sodium bicarbonate and total deprivation of sodium nitrate. B23 was stained with Nile Red, and PHB was extracted and quantified by correlating the amount of fluorescence and biopolymer concentration through spectrofluorimetry and spectrophotometry, respectively. Our results detected the production of PHB in Stigeoclonium sp. B23 and in all modified media. Treatment with increased acetate and bicarbonate and without nitrate gave the highest concentration of PHB, while the

SUBMITTER: Mourao MM 

PROVIDER: S-EPMC7761742 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature