Leaping into the Unknown World of Sporisorium scitamineum Candidate Effectors.

ABSTRACT: Sporisorium scitamineum is a biotrophic fungus causing sugarcane smut disease. In this study, we set up a pipeline and used genomic and dual transcriptomic data previously obtained by our group to identify candidate effectors of S. scitamineum and their expression profiles in infected smut-resistant and susceptible sugarcane plants. The expression profile of different genes after infection in contrasting sugarcane genotypes assessed by RT-qPCR depended on the plant genotypes and disease progression. Three candidate effector genes expressed earlier only in resistant plants, four expressed in both genotypes, and three later in susceptible plants. Ten genes were cloned and transiently expressed in N. benthamiana leaves to determine their subcellular location, while four l

SUBMITTER: Teixeira-Silva NS 

PROVIDER: S-EPMC7762069 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature