An Automated Organoid Platform with Inter-organoid Homogeneity and Inter-patient Heterogeneity.

ABSTRACT: Current organoid technologies require intensive manual manipulation and lack uniformity in organoid size and cell composition. We present here an automated organoid platform that generates uniform organoid precursors in high-throughput. This is achieved by templating from monodisperse Matrigel droplets and sequentially delivering them into wells using a synchronized microfluidic droplet printer. Each droplet encapsulates a certain number of cells (e.g., 1,500 cells), which statistically represent the heterogeneous cell population in a tumor section. The system produces >400-μm organoids within 1 week with both inter-organoid homogeneity and inter-patient heterogeneity. This enables automated organoid printing to obtain one organoid per well. The organoids recapitulate 97% gene mutations in

SUBMITTER: Jiang S 

PROVIDER: S-EPMC7762778 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature