Building a better biofilm - Formation of <i>in vivo</i>-like biofilm structures by <i>Pseudomonas aeruginosa</i> in a porcine model of cystic fibrosis lung infection.

ABSTRACT: Pseudomonas aeruginosa biofilm infections in the cystic fibrosis (CF) lung are highly resistant to current antimicrobial treatments and are associated with increased mortality rates. The existing models for such infections are not able to reliably mimic the clinical biofilms observed. We aimed to further optimise an ex vivo pig lung (EVPL) model for P. aeruginosa CF lung infection that can be used to increase understanding of chronic CF biofilm infection. The EVPL model will facilitate discovery of novel infection prevention methods and treatments, and enhanced exploration of biofilm architecture. We investigated purine metabolism and biofilm formation in the model using transposon insertion mutants in P. aeruginosa PA14 for key genes: purD, gacA a

SUBMITTER: Harrington NE 

PROVIDER: S-EPMC7762787 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature