Peptide-Based Identification of Phytophthora Isolates and Phytophthora Detection in Planta.

ABSTRACT: Phytophthora is arguably one of the most damaging genera of plant pathogens. This pathogen is well suited to transmission via the international plant trade, and globalization has been promoting its spread since the 19th century. Early detection is essential for reducing its economic and ecological impact. Here, a shotgun proteomics approach was utilized for Phytophthora analysis. The collection of 37 Phytophthora isolates representing 12 different species was screened for species-specific peptide patterns. Next, Phytophthora proteins were detected in planta, employing model plants Solanum tuberosum and Hordeum vulgare. Although the evolutionarily conserved sequences represented more than 10% of the host proteome and limited the pathogen detection,

SUBMITTER: Berka M 

PROVIDER: S-EPMC7763169 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature