The Central Region of Testican-2 Forms a Compact Core and Promotes Cell Migration.

ABSTRACT: Testicans are modular proteoglycans of the extracellular matrix of various tissues where they contribute to matrix integrity and exert cellular effects like neurite outgrowth and cell migration. Using testican-2 as a representative member of the family, we tackle the complete lack of general structural information and structure-function relationship. First, we show using isothermal titration calorimetry and modeling that extracellular calcium-binding domain (EC) has only one active calcium-binding site, while the other potential site is inactive, and that testican-2 is within extracellular matrix always in the calcium-loaded form. Next, we demonstrate using various prediction methods that N- and C-terminal regions plus interdomain connections are flexible. We support this by small-angle X-

SUBMITTER: Krajnc A 

PROVIDER: S-EPMC7763218 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature