Myeloperoxidase Modulates Hydrogen Peroxide Mediated Cellular Damage in Murine Macrophages.

ABSTRACT: Myeloperoxidase (MPO) is involved in the development of many chronic inflammatory diseases, in addition to its key role in innate immune defenses. This is attributed to the excessive production of hypochlorous acid (HOCl) by MPO at inflammatory sites, which causes tissue damage. This has sparked wide interest in the development of therapeutic approaches to prevent HOCl-induced cellular damage including supplementation with thiocyanate (SCN-) as an alternative substrate for MPO. In this study, we used an enzymatic system composed of glucose oxidase (GO), glucose, and MPO in the absence and presence of SCN-, to investigate the effects of generating a continuous flux of oxidants on macrophage cell function. Our studies show the generation of hydrogen peroxide (H2O2) by glucose and GO results

SUBMITTER: Guo C 

PROVIDER: S-EPMC7764223 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature