The Limits and Avoidance of Biases in Metagenomic Analyses of Human Fecal Microbiota.

ABSTRACT: An increasing body of evidence highlights the role of fecal microbiota in various human diseases. However, more than two-thirds of fecal bacteria cannot be cultivated by routine laboratory techniques. Thus, physicians and scientists use DNA sequencing and statistical tools to identify associations between bacterial subgroup abundances and disease. However, discrepancies between studies weaken these results. In the present study, we focus on biases that might account for these discrepancies. First, three different DNA extraction methods (G'NOME, QIAGEN, and PROMEGA) were compared with regard to their efficiency, i.e., the quality and quantity of DNA recovered from feces of 10 healthy volunteers. Then, the impact of the DNA extraction method on the bacteria identification and quantification

SUBMITTER: Bergsten E 

PROVIDER: S-EPMC7764459 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature