Epigenetic Silencing of Tumor Suppressor miR-124 Directly Supports STAT3 Activation in Cutaneous T-Cell Lymphoma.

ABSTRACT: Increasing evidence supports a potential role for STAT3 as a tumor driver in cutaneous T-cell lymphomas (CTCL). The mechanisms leading to STAT3 activation are not fully understood; however, we recently found that miR-124, a known STAT3 regulator, is robustly silenced in MF tumor-stage and CTCL cells.

Objective

We studied here whether deregulation of miR-124 contributes to STAT3 pathway activation in CTCL.

Methods

We measured the effect of ectopic mir-124 expression in active phosphorylated STAT3 (p-STAT3) levels and evaluated the transcriptional impact of miR-124-dependent STAT3 pathway regulation by expression microarray analysis.

Results

We found that ectopic expression of miR-124 results in massive downregulation of activated STAT3 in different CTCL lines, which res

SUBMITTER: Garcia-Colmenero L 

PROVIDER: S-EPMC7765332 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature