The MT1G Gene in LUHMES Neurons Is a Sensitive Biomarker of Neurotoxicity.

ABSTRACT: Identification of toxicants that underlie neurological diseases is a neglected area awaiting a valid strategy to identify such toxicants. We sought biomarkers that respond to known neurotoxicants in LUHMES immortalized neurons and evaluated these biomarkers for use in screening libraries of environmental toxicants. LUHMES immortalized human dopaminergic neurons were surveyed by RNA sequencing following challenge with parkinsonian toxicants rotenone, 6-hydroxydopamine, MPP+, and ziram (zinc dimethyldithiocarbamate; Zn²⁺DDC₂), as well as additional toxicants paraquat, MS275, and methylmercury. The metallothionein gene MT1G was the most dynamic gene expression response to all seven toxicants. Multiple toxicants also increased transcripts for SLC30A1 and SLC30A2 zinc secr

SUBMITTER: Tong ZB 

PROVIDER: S-EPMC7765730 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature