Targeted Genome Mining-From Compound Discovery to Biosynthetic Pathway Elucidation.

ABSTRACT: Natural products are an important source of novel investigational compounds in drug discovery. Especially in the field of antibiotics, Actinobacteria have been proven to be a reliable source for lead structures. The discovery of these natural products with activity- and structure-guided screenings has been impeded by the constant rediscovery of previously identified compounds. Additionally, a large discrepancy between produced natural products and biosynthetic potential in Actinobacteria, including representatives of the order Pseudonocardiales, has been revealed using genome sequencing. To turn this genomic potential into novel natural products, we used an approach including the in-silico pre-selection of unique biosynthetic gene clusters followed by their systematic heterologous expressi

SUBMITTER: Gummerlich N 

PROVIDER: S-EPMC7765855 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature