Efficiency of Recombinant CRISPR/rCas9-Mediated miRNA Gene Editing in Rice.

ABSTRACT: Drought is one of the major environmental stresses adversely affecting crop productivity worldwide. Precise characterization of genes involved in drought response is necessary to develop new crop varieties with enhanced drought tolerance. Previously, we identified 66 drought-induced miRNAs in rice plants. For the further functional investigation of the miRNAs, we applied recombinant codon-optimized Cas9 (rCas9) for rice with single-guide RNAs specifically targeting mature miRNA sequences or sites required for the biogenesis of mature miRNA. A total of 458 T₀ transgenic plants were analyzed to determine the frequency and type of mutations induced by CRISPR/rCas9 on 13 independent target miRNAs. The average mutation frequency for 13 genes targeted by single guide RNAs (sgRNAs) in

SUBMITTER: Chung PJ 

PROVIDER: S-EPMC7766165 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature