Enhanced Cell-Based Detection of Parvovirus B19V Infectious Units According to Cell Cycle Status.

ABSTRACT: Human parvovirus B19 (B19V) causes various human diseases, ranging from childhood benign infection to arthropathies, severe anemia and fetal hydrops, depending on the health state and hematological status of the patient. To counteract B19V blood-borne contamination, evaluation of B19 DNA in plasma pools and viral inactivation/removal steps are performed, but nucleic acid testing does not correctly reflect B19V infectivity. There is currently no appropriate cellular model for detection of infectious units of B19V. We describe here an improved cell-based method for detecting B19V infectious units by evaluating its host transcription. We evaluated the ability of various cell lines to support B19V infection. Of all tested, UT7/Epo cell line, UT7/Epo-STI, showed the greatest sensitivity to B19

SUBMITTER: Ducloux C 

PROVIDER: S-EPMC7766612 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature