Functional Analysis of Odorant-Binding Proteins 12 and 17 from Wheat Blossom Midge Sitodiplosis mosellana Gehin (Diptera: Cecidomyiidae).

ABSTRACT: The wheat blossom midge Sitodiplosis mosellana, one of the most disastrous wheat pests, depends highly on olfactory cues to track suitable plants. To better understand the olfactory recognition mechanisms involved in host selection, in the present study we cloned two S. mosellana adult antenna-specific odorant binding protein (OBP) genes, SmosOBP12 and SmosOBP17, and evaluated bacterially expressed recombinant proteins for their selectivity and sensitivity for host wheat volatiles using the fluorescence-based ligand binding assay. The results showed that both SmosOBPs effectively bound alcohol, ester, ketone, and terpenoid compounds. Particularly, SmosOBP12 had significantly higher affinities (K_i < 10.5 μM) than SmosOBP17 (K_i2 > 0.1 μM) to

SUBMITTER: Cheng W 

PROVIDER: S-EPMC7767053 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature