Upregulation of miR-9 and miR-193b over human Th17 cell differentiation.

ABSTRACT:

Background

Th17 cells are a newly discovered subset of CD4⁺ T cells known as key participants in various immune responses and inflammatory conditions including autoimmune diseases. Mi(cro)RNAs are a family of non-coding RNAs that regulate numerous critical immune functions. Immuno-miRNAs modulate cell biological processes in T cells, such as differentiation and function of Th17 cells. The aim of the present study is to investigate the expression of miR-9-5p, miR-193b-3p, and autoimmunity-related genes during human Th17 cells differentiation.

Methods

Human naïve CD4⁺ T cells were purified from peripheral blood mononuclear cells (PBMCs) by magnetic cell sorting system (MACS) and their purity was checked by flow-cytometric analysis. Naïve CD4⁺ T

SUBMITTER: Shirani F 

PROVIDER: S-EPMC7767567 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature