Inhibiting Protein Kinase D Promotes Airway Epithelial Barrier Integrity in Mouse Models of Influenza A Virus Infection.

ABSTRACT:

Rationale

Protein kinase D (PKD) is a serine/threonine kinase family that is involved in a wide array of signaling pathways. Although PKD has been implicated in immune responses, relatively little is known about the function of PKD in the lung or during viral infections.

Objectives

We investigated the hypothesis that PKD is involved in multiple aspects of host response to viral infection.

Methods

The selective PKD inhibitor CRT0010166 was administered to C57BL/6 mice prior to and during challenge with either inhaled double-stranded RNA or Influenza A Virus. PKD signaling pathways were investigated in human bronchial epithelial cells treated with CRT0010166, double-stranded RNA, and/or infected with Influenza A Virus.

Measurements

Total protein and albumin accum

SUBMITTER: Veazey JM 

PROVIDER: S-EPMC7767883 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature