Locus-specific analysis of DNA methylation patterns in cloned and in vitro fertilized porcine embryos.

ABSTRACT: Porcine somatic cell nuclear transfer (SCNT) is currently inefficient, as 1-3.95% of reconstructed embryos survive to term; inadequate or erroneous epigenetic reprogramming of the specialized donor somatic nucleus could be a primary reason. Therefore, a locus-specific analysis of DNA methylation dynamics in embryogenesis and the DNA methylation status of gametes and donor cells used for SCNT were conducted in the following developmentally important gene loci: POU5F1, NANOG, SOX2, H19, IGF2, IGF2R, XIST; and the retrotransposon LINE-1. There were significant epigenetic differences between the gametes and the somatic donor cells. Three gamete-specific differentially methylated regions (DMRs) in POU5F1, XIST, and LINE-1 were identified. A delayed demethylation process at POU5F1 and LINE-1 loc

SUBMITTER: Xu W 

PROVIDER: S-EPMC7768172 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature