DIA-based systems biology approach unveils E3 ubiquitin ligase-dependent responses to a metabolic shift.

ABSTRACT: The yeast Saccharomyces cerevisiae is a powerful model system for systems-wide biology screens and large-scale proteomics methods. Nearly complete proteomics coverage has been achieved owing to advances in mass spectrometry. However, it remains challenging to scale this technology for rapid and high-throughput analysis of the yeast proteome to investigate biological pathways on a global scale. Here we describe a systems biology workflow employing plate-based sample preparation and rapid, single-run, data-independent mass spectrometry analysis (DIA). Our approach is straightforward, easy to implement, and enables quantitative profiling and comparisons of hundreds of nearly complete yeast proteomes in only a few days. We evaluate its capability by characterizing changes in the yeast p

SUBMITTER: Karayel O 

PROVIDER: S-EPMC7768684 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature