Impaired Cellular Immune Responses During the First Week of Severe Acute Influenza Infection.

ABSTRACT:

Background

Cellular immune responses are not well characterized during the initial days of acute symptomatic influenza infection.

Methods

We developed a prospective cohort of human subjects with confirmed influenza illness of varying severity who presented within a week after symptom onset. We characterized lymphocyte and monocyte populations as well as antigen-specific CD8+ T-cell and B-cell responses from peripheral blood mononuclear cells using flow cytometry and enzyme-linked immunospot assays.

Results

We recruited 68 influenza-infected individuals on average 3.5 days after the onset of symptoms. Three patients required mechanical ventilation. Influenza-specific CD8+ T-cell responses expanded before the appearance of plasmablast B cells. However, the influenza-spe

SUBMITTER: Turner JS 

PROVIDER: S-EPMC7768688 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature